논문-2011, American Society for Microbiology.

AEM¹에 드디어 아들 논문이 실렸어요.

해석은 각자가 하세요.^^

 

Applied and Environmental Microbiology, March 2011, p. 2042-2050, Vol. 77, No. 6
0099-2240/11/$12.00+0     doi:10.1128/AEM.02504-10
Copyright © 2011, American Society for Microbiology. All Rights Reserved.

Characterization of a T5-Like Coliphage, SPC35, and Differential Development of Resistance to SPC35 in Salmonella enterica Serovar Typhimurium and Escherichia coli ^,

Minsik Kim and Sangryeol Ryu^*

Laboratory of Molecular Food Microbiology, Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-921, South Korea

Received 24 October 2010/ Accepted 7 January 2011

The potential of bacteriophage as an alternative biocontrol agent has recently been revisited due to the widespread occurrence of antibiotic-resistant bacteria. We isolated a virulent bacteriophage, SPC35, that can infect both Salmonella enterica serovar Typhimurium and Escherichia coli. Morphological analysis by transmission electron microscopy and analysis of its 118,351-bp genome revealed that SPC35 is a T5 group phage belonging to the family Siphoviridae. BtuB, the outer membrane protein for vitamin B₁₂ uptake, was found to be a host receptor for SPC35. Interestingly, resistant mutants of both E. coli and S. Typhimurium developed faster than our expectation when the cultures were infected with SPC35. Investigation of the btuB gene revealed that it was disrupted by the IS2 insertion sequence element in most of the resistant E. coli isolates. In contrast, we could not detect any btuB gene mutations in the resistant S. Typhimurium isolates; these isolates easily regained sensitivity to SPC35 in its absence, suggesting phase-variable phage resistance/sensitivity. These results indicate that a cocktail of phages that target different receptors on the pathogen should be more effective for successful biocontrol.

 

---

* Corresponding author. Mailing address: Department of Food and Animal Biotechnology, Seoul National University, Shilim Dong, Seoul 151-921, South Korea. Phone: 82-2-880-4856. Fax: 82-2-873-5095. E-mail: sangryu@snu.ac.kr

 

Published ahead of print on 21 January 2011.

Supplemental material for this article may be found at http://aem.asm.org/.

 

---

Applied and Environmental Microbiology, March 2011, p. 2042-2050, Vol. 77, No. 6
0099-2240/11/$12.00+0     doi:10.1128/AEM.02504-10
Copyright © 2011, American Society for Microbiology. All Rights Reserved.

 

똘지, 축하해.

언제나 널 믿어주는 영원한 후원자인 엄마가.

1. AEM이란? 미국 미생물학회에서 발간하는 응용환경미생물지, 미국 미생물학회에서 온라인 및 인쇄로 발행하는 학술저널 [본문으로]